RNA

Part:BBa_K5102039:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


ProgRAM recording tape 2.2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The tape features a series of START codons in Kozak consensus context that are placed in three consecutive forward open reading frames. The nucleotides in the variable protospacer regions were strategically selected to optimize both the guide RNAs (gRNAs) necessary for writing on the tape, as well as the embedded translation initiation elements (Kozak sequences) for reading.



Source

This part was ordered as a synthetic oligo from a DNA synthesis provider.

References